|
|
Services |
Amount |
h |
Non-viral services
 |
Plasmid midi-prep |
|
$475.00 |
Plasmid maxi-prep |
|
$1,000.00 |
Transformation |
|
$100.00 |
Plasmid cloning |
|
$1,000.00 |
Stock plasmids
click here for available stocks |
|
|
|
Viral Services
|
Lentiviral construct |
|
$1,000.00 |
Lentiviral production |
30 ml
(106 particles/ml) |
$300.00 |
Lentiviral production-small scale(concentrated) |
100 µl
(109 particles/ml) |
$1,000.00 |
Lentiviral production-large scale(concentrated) |
500 µl
(109 particles/ml) |
$3,500.00 |
Lentiviral stock aliquots
click here for available stocks |
20 µl
(109particles/ml) |
$250.00 |
Titration
(fluorescent protein -expressing vectors) |
|
$100.00 |
Titration by P24 assay
(non-fluorescent protein -expressing vectors) |
|
$500.00 |
RCA/P24 ELISA |
|
$1,000.00 |
Serial transfer for safety assay |
|
$1,000.00 |
|
Transgenesis
|
DNA purification for microinjection |
|
$200.00 |
Pronuclear injection for generating transgenic mice |
2 founders |
$3,500.00 |
Zona lentiviral Injection for generating transgenic mice |
3 founders |
$3,000.00 |
Zona lentiviral Injection for generating transgenic rats |
3 founders |
$5,000.00 |
Genotyping by PCR |
|
$500.00 |
|
Knockout
|
Gene targeting construct |
|
$5,000.00 |
ES cell electroporation |
|
$1,000.00 |
ES cell colonies picking |
200 colonies |
$1,000.00 |
Southern blot for screening including gDNA extraction |
|
$2,000.00 |
Blastocyst injection |
2 transfers |
$1,000.00 |
|
Knockdown
|
lenti-polymerase II knockdown construct(miRNA) |
|
$1,000.00 |
lenti-polymerase III knockdown construct(shRNA) |
|
$1,000.00 |
|
Animal Bioassays
|
Teratoma analysis (Scid mice) |
4 injections |
$800.00 |
Chimera analysis |
2 transfers |
$1,000.00 |
|
Cell lines
|
293FT |
|
$100.00 |
Mouse ES R1 |
|
$300.00 |
HT1080 |
|
$100.00 |
Hela |
|
$50.00 |
|
|
