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In vivo imagingThe imaging core provides total body non-invasive functional imaging for PDC investigators. The core is responsible for tracking cell lineage contributions of ES and EG chimeric embryos and the fates of transplanted hES cells. The core also uses high resolution MRI to map fetal development and teratoma formation, image excised teratomas prior to sectioning, and track transplanted embryonic and adult stem cells in vivo. At the whole animal level, we will utilize new small animal imaging technologies such as MRI and PET, to perform longitudinal studies of pregnant monkeys and monitor viability and phenotypic differences of the chimeric fetus and placenta throughout pregnancy.
Figure 1. PET scan of a six month old rhesus macaque using a [C-11]diprenorphine tracer, specific for opioid receptors. PET brain imaging is performed by the veterinarian as a diagnostic measure for the normalcy of chimeric offspring.
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The
imaging core, directed by Dr. Chris Navara, provides exceptional
high-sensitivity detection of stem cell and developmental dynamics
in vitro, in utero and in vivo. We will combine the strengths
of in vivo non-invasive MRI with in vitro imaging by conventional,
confocal, and electron microscopy. The functions of the Imaging
Core will be performed at three locations: MRI will be performed
at the NIBIB-funded Pittsburgh NMR Center for Biomedical Research
at Carnegie Mellon University (headed by Dr. Eric Ahrens); EM
studies will be performed at the Center for Biologic Imaging at
the University of Pittsburgh (headed by Dr. Simon Watkins); and
cellular imaging using the sophisticated light microscopic instruments
at the Pittsburgh Development Center. These facilities are designed
for the purpose of providing state of the art non-invasive imaging
technologies to biomedical researchers. 
