Research in the Orwig lab focuses on the biological activity and functional genetic characterization of male germline stem cells. Stem cells have become the focus of intense investigation due to their essential role in embryonic and adult tissue development and their capacity to regenerate unhealthy or damaged tissues.

Male germline stem cells lie at the foundation of spermatogenesis, which is one of the most productive self-renewing systems in adult animals (a man produces about 1000 spermatozoa every time his heart beats). In addition, spermatogonial stem cells are unique among adult tissue stem cells because they can pass genes through the germline to subsequent generations. Investigation of these undifferentiated cells provides insight into several aspects of mammalian biology including, stem cell/niche interactions, germline development, male infertility, transgenesis, and gene therapy.