<%@LANGUAGE="JAVASCRIPT" CODEPAGE="1252"%> Orwig Laboratory-Publications
Magee Womens Research Institute University of Pittsburgh

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  1. B.P. Hermann, M. Sukhwani, F. Winkler, J.N. Pascarella, K.A. Peters, Y. Sheng, H. Valli, M. Rodriguez, M. Ezzelarab, G. Dargo, K. Peterson, K. Masterson, C. Ramsey, T. Ward, M. Lienesch, A. Volk, D.K. Cooper, A.W. Thomson, J.E. Kiss, M.C. Penedo, G.P. Schatten, S. Mitalipov, K.E. Orwig. 2012. Spermatogonial stem cell transplantation into rhesus testes regenerates spermatogenesis producing functional sperm. Cell Stem Cell 11(5):715-26. [PUBMED]
  2. C.A. Easley 4th, B.T. Phillips, M.M. McGuire, J.M. Barringer, H. Valli, B.P. Hermann, C.R. Simerly, A. Rajkovic, T. Miki, K.E. Orwig, G.P. Schatten. Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells. 2012. Cell Rep. 2(3):440-6. [PUBMED]
  3. H. Suzuki, H.W. Ahn, T. Chu, W. Bowden, K. Gassei, K. Orwig, A. Rajkovic. 2012. SOHLH1 and SOHLH2 coordinate spermatogonial differentiation. Dev Biol. 361(2):301-12. [PUBMED]
  4. A.T. Clark, B.T. Phillips, K.E. Orwig. 2011. Fruitful progress to fertility: male fertility in the test tube. Nat Med. 17(12):1564-5. [PUBMED]
  5. B.P. Hermann, M. Sukhwani, J. Salati, Y. Sheng, T. Chu, K.E. Orwig. 2011. Separating spermatogonia from cancer cells in contaminated prepubertal primate testis cell suspensions. Hum Reprod. 26(12):3222-31. [PUBMED]
  6. B.P. Hermann, B.T. Phillips, K.E. Orwig. 2011. The elusive spermatogonial stem cell marker? Biol Reprod. Aug;85(2):221-3. [PUBMED]
  7. M.E. Ochalski, J.J. Shuttleworth, T. Chu T, K.E. Orwig. 2011. Thalidomide treatment attenuates chemotherapy-induced gonadal toxicity. Fertil Steril. 95:819-822. [PUBMED]

  8. A. Honaramooz, S. Schlatt, K. Orwig, N.H. Kim. Recent advances in reproductive technologies. 2011. Vet Med Int. 915031. Epub 2011 Jun 22 .[PUBMED]
  9. C. Simerly, D. McFarland, C. Castro, C.C. Lin, C. Redinger, E. Jacoby, J. Mich-Basso, K. Orwig, P. Mills, E. Ahrens, C. Navara, G. Schatten. 2011. Interspecies chimera between primate embryonic stem cells and mouse embryos: monkey ESCs engraft into mouse embryos, but not post-implantation fetuses. Stem Cell Res. 7(1):28-40. [PUBMED]
  10. B.T. Phillips, K. Gassei , K.E. Orwig. 2010. Spermatogonial stem cell regulation and spermatogenesis. Phil Trans R Soc B ; 365, 1663-1678. [PUBMED]
  11. A. Ben-Yehudah, C.A. Easley 4th, B.P. Hermann, C. Castro, C. Simerly, K.E. Orwig, S. Mitalipov, G. Schatten. 2010. Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns.Stem Cell Res Ther. 1(3):24. [PUBMED]
  12. B.P. Hermann, M. Sukhwani, M.C. Hansel, K.E. Orwig. 2010. Spermatogonial stem cells in higher primates: are there differences from those in rodents? Reproduction 139: 479–493; DOI: 10.1530/REP-09-0255 [ABSTRACT]
  13. Y. Sheng, C.C. Lin, J. Yue, M. Sukhwani, J.J. Shuttleworth, T. Chu, K.E. Orwig. Generation and characterization of a Tet-On (rtTA-M2) transgenic rat. 2010. BMC Developmental Biology, 10:17. [ABSTRACT]
  14. B.P. Hermann, M. Sukhwani, D.R. Simorangkir, T. Chu, T.M. Plant, K.E. Orwig. 2009. Molecular Dissection of the Male Germ Cell Lineage Identifies Putative Spermatoganial Stem Cells in Rhesus Macaques. Hum Reprod. 24(7): 1704-16. [PUBMED]
  15. R. Escamilla-Hernandez, L. Little-Ihrig, K.E. Orwig , J. Yue, U. Chandran, A.J. Zeleznik. 2008. Constitutively Active Protein Kinase A Qualitatively Mimics the Effects of Follicle-Stimulating Hormone on Granulosa Cell Differentiation. Mol Endocrinol. 22(8):1842-52. [PUBMED]
  16. K.E. Orwig, B.Y. Ryu, S. Master, B.T. Phillips, M. Mack, M.R. Avarbock, R.L. Brinster. 2008. Genes Involved in Post-transcriptional Regulation are Over Represented in Stem/progenitor Spermatogonia of Cryptorchid Mouse Testes. Stem Cells. 26(4):927-38. [PUBMED]
  17. J. Yue, Y. Sheng, K.E. Orwig. 2008. Identification of Novel Homologous microRNA Genes in the Rhesus Macaque Genome. BMC Genomics. 9(1): 8. [PUBMED]
  18. B.P. Hermann, M. Sukhwani, C.C. Lin, Y. Sheng, J. Tomko, M. Rodriguez, J.J. Shuttleworth, D. McFarland, R.M. Hobbs, P.P. Pandolfi, G.P. Schatten, K.E. Orwig. 2007. Characterization, Cryopreservation and Ablation of Spermatogonial Stem Cells In Adult Rhesus Macaques. Stem Cells 25(9):2330-2338. [PUBMED]
  19. Navara CS, Mich-Basso JD, Redinger CJ, Ben-Yehudah A, Jacoby E, Kovkarova-Naumovski E, Sukhwani M, Orwig K, Kaminski N, Castro CA, Simerly CR, Schatten G. 2007. Pedigreed Primate Embryonic Stem Cells, Express Homogeneous Familial Gene Profiles. Stem Cells. 25(11):2695-704. [PUBMED]
  20. B.Y. Ryu, K.E. Orwig, J.M. Oatley, C.C. Lin, L.J. Chang, M.R. Avarbock, R.L. Brinster. 2007. Efficient Generation of Transgenic Rats Through the Male Germline using Lentiviral Transduction and Transplantation of Spermatogonial Stem Cells. J Androl. 28(2):353-60. [PUBMED]
  21. B.Y. Ryu, K.E. Orwig, J.M. Oatley, M.R. Avarbock, R.L. Brinster. 2006. Effects of Aging and Niche Microenvironment on Spermatogonial Stem Cell Self-Renewal.  Stem Cells. 24(6):1505-1511. [PUBMED]
  22. A. Buageaw, M. Sukhwani, A. Ben-Yehudah, J Ehmcke, VY Rawe, C Pholpramool, K.E. Orwig, S. Schlatt. 2005. GDNF Family Receptor alpha1 Phenotype of Spermatogonial Stem Cells in Immature Mouse Testes. Biol. Reprod. 73(5):1011-1016. [PUBMED]
  23. K.E. Orwig, S. Schlatt. 2005. Cryopreservation and transplantation of spermatogonia and testicular tissue for preservation of male fertility.
    J Natl Cancer Inst Monogr. 34:51-56. [PUBMED]
  24. B.Y Ryu, K.E. Orwig, H. Kubota, M.R. Avarbock, R.L. Brinster. 2004. Phenotypic and functional characteristics of spermatogonial stem cells in rats. Dev. Biol. 274:158-170. [PUBMED]
  25. J.A. Costoya , R.M. Hobbs, M. Barna, G. Cattoretti, K. Manova,
    M. Sukhwani, K.E. Orwig, D.J. Wolgemuth, P.P. Pandolfi. 2004. Essential role of Plzf in maintenance of spermatogonial stem cells. Nature Genetics. 36: 653-659. [PUBMED]
  26. B.Y. Ryu, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2003. Stem cell and niche development in the postnatal rat testis. Dev. Biol. 263:253-263. [PUBMED]
  27. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2003. Restoration of spermatogenesis in infertile mice by Sertoli cell transplantation. Biol. Reprod. 68:1064-1071. [PUBMED]
  28. C.J. Brinster, B.-Y. Ryu, L. Karagenc, R.L. Brinster, K.E. Orwig. 2003. Restoration of fertility by germ cell transplantation requires effective recipient preparation. Biol. Reprod. 69:412-420. [PUBMED]
  29. K.E. Orwig, B.-Y. Ryu, M.R. Avarbock, R.L. Brinster. 2002. Male germ-line stem cell potential is predicted by morphology of cells in neonatal rat testes. Proc. Nat. Acad. Sci. USA. 99:11706-11711. [PUBMED]
  30. K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2002. Retrovirus-mediated modification of male germline stem cells in rats. Biol. Reprod. 67:874-879. [PUBMED]
  31. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2002. Germ line stem cell competition in postnatal mouse testes. Biol. Reprod. 66:1491-1497. [PUBMED]
  32. K.E. Orwig, T. Shinohara, M.R. Avarbock, R.L. Brinster. 2002. Functional analysis of stem cells in the adult rat testis. Biol. Reprod. 66:944-949. [PUBMED]
  33. M. Nagano, C.J. Brinster, K.E. Orwig, B.-Y. Ryu, M.R. Avarbock, R.L. Brinster. 2001. Transgenic mice produced by retroviral transduction of male germ-line stem cells. Proc. Nat. Acad. Sci. USA. 98:13090-13095. [PUBMED]
  34. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2001. Remodeling of the postnatal mouse testis is accompanied by dramatic changes in stem cell number and niche accessibility. Proc. Nat. Acad. Sci. USA. 98:6186-6191. [PUBMED]
  35. T. Shinohara, K.E. Orwig, M.R. Avarbock, R.L. Brinster. 2000. Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells. Proc. Nat. Acad. Sci. USA. 97:8346-8351. [PUBMED]
  36. S. Filley, S. Supansic, U. Salli, K. Orwig, F. Stormshak. 2000. Myristoylated alanine-rich c kinase substrate protein and mRNA in bovine corpus luteum during the estrous cycle. Endocrine 12:289-294. [PUBMED]
  37. K.E. Orwig and M.J. Soares. 1999. Transcriptional activation of the decidual/trophoblast prolactin-related protein gene. Endocrinology. 140:4032-4039. [PUBMED]
  38. G.D. Dai, B.M. Chapman, B. Liu, K.E. Orwig, D. Wang, R.A. White, B. Preuett, M.M. Soares. 1998. A new member of the mouse prolactin (PRL)-like protein-C subfamily, PRL-like protein-Ca: Structure and Expression. Endocrinology 139:5157-5163. [PUBMED]
  39. K.E. Orwig, M.W. Wolfe, C.B. Cohick, G. Dai, T.J. Peters, M.J. Soares. 1998. Trophoblast-specific regulation of endocrine-related genes. Trophoblast Research 12:65-85.
  40. H. Müller, K.E. Orwig, M.J. Soares. 1998. Identification of two new members of the mouse prolactin gene family. Biochim. Biophys. Acta 1396:251-258. [PUBMED]
  41. M.J. Soares, H. Müller, K.E. Orwig, T.J. Peters, G. Dai. 1998. The Uteroplacental prolactin family and pregnancy. Biol. Reprod. 58:273-284. [PUBMED]
  42. H. Müller, R. Ishimura, K.E. Orwig, B. Liu, M.J. Soares. 1998. Homologues for prolactin-like protein-A and B are present in the mouse. Biol. Reprod. 58:45-51. [PUBMED]
  43. K.E. Orwig, R. Ishimura, H. Müller, B. Liu and M.J. Soares. 1997. Identification and characterization of a mouse homolog for decidual/trophoblast prolactin-related protein. Endocrinology 138:5511-5517. [PUBMED]
  44. K.E. Orwig, C.A. Rasmussen and M.J. Soares. 1997. Decidual Signals in the establishment of pregnancy: the prolactin family. Trophoblast Research 10:329-343.
  45. K.E. Orwig, G. Dai, C.A. Rasmussen and M.J. Soares. 1997. Decidual/trophoblast prolactin-related protein: characterization of gene structure and cell-specific expression. Endocrinology 138:2491-2500. [PUBMED]
  46. C.A. Rasmussen, K.E. Orwig, S. Vellucci and M.J. Soares. 1997. Dual expression of prolactin-related protein in decidua and trophoblast tissues during pregnancy. Biol. Reprod. 56:647-654. [PUBMED]
  47. C.A. Rasmussen, K. Hashizume, K.E. Orwig, L. Xu and M.J. Soares. 1996. Decidual prolactin-related protein: heterologous expression and characterization. Endocrinology 137:5558-5566. [PUBMED]
  48. M.J. Soares, B.M. Chapman, C.B. Cohick, C.A. Rasmussen, G. Dai, T. Kamei and K.E. Orwig. 1996. Differentiation of trophoblast endocrine cells. Placenta 17:277-289. [PUBMED]
  49. F. Stormshak, K.E. Orwig and J.E. Bertrand. 1995. Dynamics of molecular mechanisms underlying ovarian oxytocin secretion. Journal of Reproduction and Fertility. 49:379-390. [PUBMED]
  50. K.E. Orwig, J.E. Bertrand, B.-R.Ou, N.E. Forsberg and F. Stormshak. 1994. Immunochemical characterization and cellular distribution of protein kinase C isozymes in the bovine corpus luteum. Comparative Biochemistry and Physiology. 108B:53-57. [PUBMED]
  51. K.E. Orwig, J.E. Bertrand, B.-R.Ou, N.E. Forsberg and F.Stormshak. 1994. Involvement of protein kinase C, calpains and calpastatin in prostaglandin F2*-induced oxytocin secretion from the bovine corpus luteum. Endocrinology. 134:78-83. [PUBMED]
  52. K.E. Orwig, S. Leers-Sucheta, M.F. Moghaddam, Z.D. Jiang, W.R. Gerwick and F. Stormshak. 1992. Unique metabolites of eicosapentaenoic acid interfere with corpus luteum function in the ewe. Prostaglandins. 44:519-530. [PUBMED]

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